Efficacy of biological therapies against onion basal rot caused by Fusarium oxysporum f. sp. cepae under field and storage conditions
Keywords:
Allium cepa, basal rot, antagonism, Fusarium oxysporum f. sp. cepae, yeastsAbstract
Basal rot disease of onion is caused by Fusarium oxysporum f. sp. cepae (Hans.) (FOC) economically significant losses wherever onion is grown. Fusarium oxysporum were isolated from diseased onion cultivated in different places of Assiut, Egypt. Efficacy of certain yeasts was evaluated for controlling the basal rot of onion in vitro. Among of the tested isolates, Saccharomyces cerevisiae gave the greatest inhibition (57.74%) and Candida tropicalis (1) significantly exerted the greatest reduction of mycelial growth of F. oxysporum f. sp. cepae (51.18%). Based on the in vitro screening, effective yeasts were evaluated under greenhouse, field and storage conditions. Yeasts were applied by two methods (add the pathogen and antagonistic yeasts to soil before sowing seedling onion, and seedling onion soaking in yeasts for 20 minute). Both methods of inoculation showed substantial impact on disease development and plant growth. Add method caused maximum reduction in plant germination, followed by soaking method as compared to control. Application of fungicide (Captain) as compared brought a remarkable increase in seedling emergence of treated plants inoculated with F. oxysporum as compared to the untreated plants. In conclusion, tested yeasts were useful as an alternative to chemical control of the onion basal rot and to enhanced growth and yield of onion.
Metrics
Metrics Loading ...
References
Abdel-Kader MM, El-Mougy Nehal S, Aly Lashin MDE, 2012. Different approaches of bio-control agents for controlling root rot incidence of some vegetables under greenhouse conditions. International Journal of Agriculture and Forestry 2(1): 115–127.
Anbukkasari V, 2013, Studies on pre and post harvest treatments for extending
shelf life of onion (Allium cepa L.var aggregatum don) cv. Co on 5. Ph.D.
Thesis, Department of Vegetable Crops, Tamilnadu Agriculture University, Coimbatore, India.
Astasa G, Aliad A, 2005. Biological control of Fusarium wilt of Abaca (Fusarium oxysporum) with Trichoderma and yeast. Philippine Journal of Crop Science 30(2): 29–37.
Baker KF, Cook RJ, 1974. Biological control of plant pathogens. WH Freeman & Co., San Francisco, California, USA, 433 pp.
Barnett JA, Payne RW, Yarrow D, 2000, Yeasts: characteristics and identification. 3rd edition, Cambridge University Press, London, UK.
Booth CC, 1985, The genus Fusarium. 2nd ed., Commonwealth Mycological Institute, Kew, Surrey, England.
Coskuntuna A, Ozer N, 2008, Biological control of onion basal rot disease using Trichoderma harzianum and induction of antifungal compounds in onion set fallowing seed treatment. Crop Protection 27: 330–336.
Cramer CS, 2000. Breeding and genetics of Fusarium basal rot resistance in onion. Euphytica 115: 159–166.
Davis RM, 2008. Fusarium oxysporum f. sp. cepae causing onion and garlic basal rot. Plant Pathology, UC Pest Management Guidelines, University of California, USA.
Dewedar GA, Ibrahim EAM, 2016. Effect of application of yeast on yield and seed quality of some rice cultivars. Journal of Plant Production 7(6): 593–601.
Dissanayake MLMC, Kashima R, Tanaka S, Ito Sh, 2009. Pathogenic variation and molecular characterization ofFusariumspecies isolated from wilted welsh onion in Japan. Journal of General Plant Pathology 75 (1) :37–45.
El-mehalawy AA, Hassanein Naziha M, Khater Hend M, Karam El-din El-zahraa A, Youssef YA, 2004. Influence of maize root colonization by the rhizosphere actinomycetes and yeast fungi on plant growth and on the biological control of late wilt disease. International Journal of Agriculture and Biology 6(4): 599–605.
Freimoser FM, Rueda Mejia MP, Tilocca B, Migheli Q, 2019. Biocontrol yeasts: mechanisms and applications. World Journal of Microbiology and Biotechnology 35: 154.
Freimoser FM, Rueda Mejia MP, Tilocca B, Migheli Q, 2019. Biocontrol yeasts: mechanisms and applications. World Journal of Microbiology and Biotechnology 35: 154.
Gerlach W, Nirenberg H, 1982. The genus Fusarium-A pictorial atlas. Mitteilungen aus der Biologischen Bundesanstalt Für Land- und Forstwirtschaft (Berlin - Dahlem) 209:1–405.
Gomez KA, Gomez AA, 1984. Statistical Procedures for Agricultural Research, 2nd Ed., John Willey, New York, USA, 680 pp.
Hoflich G, Kühn G, 1996. Promotion of plant growth and nutrient uptake of cruciferous oil and intercrops by inoculated rhizosphere microorganisms. Zeitscrift fur Pflanzenernährung und Bodenkunde 159: 575–81.
Joubert PM, Doty SL, 2018. Endophytic Yeasts: Biology, Ecology and Applications. Endophytes of Forest Trees, Forestry Sciences, Volume86, Springer, USA.
Kamel SM, Morsy Ebtsam M, Massoud ON, 2016. Potentiality of some yeast species as biocontrol agents against Fusarium oxysporum f. sp. cucumerinum the causal agent of cucumber wilt. E. J. of B. Pest Control 26(2): 185–193
Keusgen M, 2002. Health and Alliums. In Rabinowitch HD and Currah L (Eds.), Allium Crop Science: Recent Advances. CABI Publishing, Wallingford, UK, pp. 357–378.
Kumar V, Sharma SK, Sagar NA, 2015. Post harvest management of fungal diseases in onion - A Review. International Journal of Current Microbiology and Applied Sciences 4(6): 737–752.
Leslie JF, Summerell BA, 2006. The Fusarium laboratory manual. Blackwell Professional, Ames, Iowa, USA.
Nguyen MT, Ranamukhaarachchi SL, Hannaway DB, 2011. Efficacy of antagonist strains of Bacillus megaterium, Enterobacter cloacae, Pichia uillermondii and Candida ethanolica against bacterial wilt disease of tomato. Journal of Phytology 3(2): 01–10
Ozer N, Koycu ND, Chilosi G, Pizzuolu PH, Coskuntuna A and Magro P, 2003. Pectolytic isoenzymes by Fusarium oxysporum f. sp. cepae and antifungal compounds in onion cultivars as a response to pathogen infection. Canadian Journal of Plant Pathology 25: 249–57.
Rangaswami G, 1958. An agar blocks technique for isolating soil microorganisms with special reference to pythiaceous fungi. Scientific Culture 24: 85.
Saxena A, 2007. Screening of onion cultivars for Fusarium basal rot and spatial distribution of Fusarium oxysporum f. sp. cepae. M.Sc Thesis, New Mexico State University, LAS Cruces, NM, USA.
Schroeder BK, du Toit LJ, 2010. Effects of postharvest onion curing parameters on Enterobacter bulb decay in storage. Plant Disease 94: 1425–1430.
Schwartz HF, 2010, Soil-borne diseases of onion, Fact Sheet no. 2.940, Colorado State University, USA, pp. 1–5.
Shalaby ME, El-Nady MF, 2008. Application of Saccharomyces cerevisiae as a biocontrol agent against Fusarium infection of sugar beet plants. Acta Biologica Szegediensts 52(2): 271–275.
Sudhasha S, Usharani S, Ravimycin T, 2008, Surveillance of onion basal rot disease incidence caused by Fusarium oxysporum f. sp. cepae and varietal reaction under field condition. Asian Journal of Biological Sciences 3(2): 369–37.
van der Walt JP, Yarrow D, 1984. The genus Arxiozyma gen. nov. (Saccharomycetaceae). South African Journal of Botany 3: 340–342
Zeidan R, Ul-Hassan Z, Al-Thani R, Balmas V, Jaoua S, 2018. Application of Low-Fermenting Yeast Lachancea thermotolerans for the Control of Toxigenic Fungi Aspergillus parasiticus, Penicillium verrucosum and Fusarium graminearum and their mycotoxins. Toxins 10(6): 242.
Anbukkasari V, 2013, Studies on pre and post harvest treatments for extending
shelf life of onion (Allium cepa L.var aggregatum don) cv. Co on 5. Ph.D.
Thesis, Department of Vegetable Crops, Tamilnadu Agriculture University, Coimbatore, India.
Astasa G, Aliad A, 2005. Biological control of Fusarium wilt of Abaca (Fusarium oxysporum) with Trichoderma and yeast. Philippine Journal of Crop Science 30(2): 29–37.
Baker KF, Cook RJ, 1974. Biological control of plant pathogens. WH Freeman & Co., San Francisco, California, USA, 433 pp.
Barnett JA, Payne RW, Yarrow D, 2000, Yeasts: characteristics and identification. 3rd edition, Cambridge University Press, London, UK.
Booth CC, 1985, The genus Fusarium. 2nd ed., Commonwealth Mycological Institute, Kew, Surrey, England.
Coskuntuna A, Ozer N, 2008, Biological control of onion basal rot disease using Trichoderma harzianum and induction of antifungal compounds in onion set fallowing seed treatment. Crop Protection 27: 330–336.
Cramer CS, 2000. Breeding and genetics of Fusarium basal rot resistance in onion. Euphytica 115: 159–166.
Davis RM, 2008. Fusarium oxysporum f. sp. cepae causing onion and garlic basal rot. Plant Pathology, UC Pest Management Guidelines, University of California, USA.
Dewedar GA, Ibrahim EAM, 2016. Effect of application of yeast on yield and seed quality of some rice cultivars. Journal of Plant Production 7(6): 593–601.
Dissanayake MLMC, Kashima R, Tanaka S, Ito Sh, 2009. Pathogenic variation and molecular characterization ofFusariumspecies isolated from wilted welsh onion in Japan. Journal of General Plant Pathology 75 (1) :37–45.
El-mehalawy AA, Hassanein Naziha M, Khater Hend M, Karam El-din El-zahraa A, Youssef YA, 2004. Influence of maize root colonization by the rhizosphere actinomycetes and yeast fungi on plant growth and on the biological control of late wilt disease. International Journal of Agriculture and Biology 6(4): 599–605.
Freimoser FM, Rueda Mejia MP, Tilocca B, Migheli Q, 2019. Biocontrol yeasts: mechanisms and applications. World Journal of Microbiology and Biotechnology 35: 154.
Freimoser FM, Rueda Mejia MP, Tilocca B, Migheli Q, 2019. Biocontrol yeasts: mechanisms and applications. World Journal of Microbiology and Biotechnology 35: 154.
Gerlach W, Nirenberg H, 1982. The genus Fusarium-A pictorial atlas. Mitteilungen aus der Biologischen Bundesanstalt Für Land- und Forstwirtschaft (Berlin - Dahlem) 209:1–405.
Gomez KA, Gomez AA, 1984. Statistical Procedures for Agricultural Research, 2nd Ed., John Willey, New York, USA, 680 pp.
Hoflich G, Kühn G, 1996. Promotion of plant growth and nutrient uptake of cruciferous oil and intercrops by inoculated rhizosphere microorganisms. Zeitscrift fur Pflanzenernährung und Bodenkunde 159: 575–81.
Joubert PM, Doty SL, 2018. Endophytic Yeasts: Biology, Ecology and Applications. Endophytes of Forest Trees, Forestry Sciences, Volume86, Springer, USA.
Kamel SM, Morsy Ebtsam M, Massoud ON, 2016. Potentiality of some yeast species as biocontrol agents against Fusarium oxysporum f. sp. cucumerinum the causal agent of cucumber wilt. E. J. of B. Pest Control 26(2): 185–193
Keusgen M, 2002. Health and Alliums. In Rabinowitch HD and Currah L (Eds.), Allium Crop Science: Recent Advances. CABI Publishing, Wallingford, UK, pp. 357–378.
Kumar V, Sharma SK, Sagar NA, 2015. Post harvest management of fungal diseases in onion - A Review. International Journal of Current Microbiology and Applied Sciences 4(6): 737–752.
Leslie JF, Summerell BA, 2006. The Fusarium laboratory manual. Blackwell Professional, Ames, Iowa, USA.
Nguyen MT, Ranamukhaarachchi SL, Hannaway DB, 2011. Efficacy of antagonist strains of Bacillus megaterium, Enterobacter cloacae, Pichia uillermondii and Candida ethanolica against bacterial wilt disease of tomato. Journal of Phytology 3(2): 01–10
Ozer N, Koycu ND, Chilosi G, Pizzuolu PH, Coskuntuna A and Magro P, 2003. Pectolytic isoenzymes by Fusarium oxysporum f. sp. cepae and antifungal compounds in onion cultivars as a response to pathogen infection. Canadian Journal of Plant Pathology 25: 249–57.
Rangaswami G, 1958. An agar blocks technique for isolating soil microorganisms with special reference to pythiaceous fungi. Scientific Culture 24: 85.
Saxena A, 2007. Screening of onion cultivars for Fusarium basal rot and spatial distribution of Fusarium oxysporum f. sp. cepae. M.Sc Thesis, New Mexico State University, LAS Cruces, NM, USA.
Schroeder BK, du Toit LJ, 2010. Effects of postharvest onion curing parameters on Enterobacter bulb decay in storage. Plant Disease 94: 1425–1430.
Schwartz HF, 2010, Soil-borne diseases of onion, Fact Sheet no. 2.940, Colorado State University, USA, pp. 1–5.
Shalaby ME, El-Nady MF, 2008. Application of Saccharomyces cerevisiae as a biocontrol agent against Fusarium infection of sugar beet plants. Acta Biologica Szegediensts 52(2): 271–275.
Sudhasha S, Usharani S, Ravimycin T, 2008, Surveillance of onion basal rot disease incidence caused by Fusarium oxysporum f. sp. cepae and varietal reaction under field condition. Asian Journal of Biological Sciences 3(2): 369–37.
van der Walt JP, Yarrow D, 1984. The genus Arxiozyma gen. nov. (Saccharomycetaceae). South African Journal of Botany 3: 340–342
Zeidan R, Ul-Hassan Z, Al-Thani R, Balmas V, Jaoua S, 2018. Application of Low-Fermenting Yeast Lachancea thermotolerans for the Control of Toxigenic Fungi Aspergillus parasiticus, Penicillium verrucosum and Fusarium graminearum and their mycotoxins. Toxins 10(6): 242.
Published
2021-11-28
How to Cite
Ahmed, H. A. M., Soliman, Z., Khalil, M. A., & Fawaz, S. B. M. (2021). Efficacy of biological therapies against onion basal rot caused by Fusarium oxysporum f. sp. cepae under field and storage conditions. Journal of Phytopathology and Disease Management, 8(1), 92–105. Retrieved from https://www.ppmj.net/index.php/ppmj/article/view/226
Issue
Section
Research Articles
License
Authors who publish with Journal of Phytopathology and Disease Management agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under the Creative Commons Attribution-Non Commercial License (CC BY-NC). This allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Archives of Agricultural Sciences Journal is an Open Access Journal, and articles published are distributed under the terms of the Creative Commons Attribution-Non Commercial License (CC BY-NC). Readers may copy, distribute, and display the work for non commercial purposes with the proper citation of the original work. However, the journal retains the right to exploit subsidiary rights on behalf of the authors.
- Authors are able to enter into separate, additional contractural arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process with full disclosure to the journal, as it can lead to productive exchanges, as well as earlier and greater citation of published work. Following publication in Archives of Agricultural Sciences Journal, the author should update the repository, and include a citation and link to the published work.
Click here for more information on Licensing policy
Print ISSN: 3009-6111.png)
