Biological control of damping-off and root rot of fenugreek

Abstract

Eight Trichoderma and nine bacterial isolates which isolated from rhizospher and nodules of fenugreek plants. Also four  isolates of rhizobacteria (PGPR) namely Basillus subtilis (B.s), Basillus polymyxa (B.p), Basillus megaterium (B.m) and Pseudomonas fluorescens (P.f ) were tested in vitro for thir ability against Fusarium solani, Rhizoctonia solani and Macrophomina phaseolina which caused damping-off and root rot of fenugreek plants.The results showed that  Trichoderma isolate number (T3) gave  the highest reduction on maycelial growth of three pathogenic  fungi followed by isolate number (T2) which adentified as Trichoderma harzianum and Trichoderma hamatum, respectively. Pseudomonas fluorescens followed by Basillus polymyxa , Rhizobium sp. isolate (Rh3), Basillus subtilis and Basillus megaterium gave highly antagonistic effect was clear against the tested fungi as will as used in greenhous experiment. A pot experiment was carried out under greenhouse conditions. Results showed that Treated seed of fenugreek caltivar (Giza 2 cv.)  with Rhizobacteria and or  treated soil with T. harzianum and T. hamatum reduced pre and post damping-off  and root rot diseases of fenugreek and increased survival plant compared with the control. Trichoderma harzianum followed by Rhizobium sp. isolate (Rh3) gave the best reduction in these respects.